His tag Purification

His tags are used widely to label proteins for a wide range of functions. The His-tag on the protein is attracted to the metal ion on the agarose bead, to assist in separation. Several companies offer Nickel activate chelating beads and then the laboratory personnel have to adapt their protein production to suit the beads.
ABT took a different approach and they offer a comprehensive range of beads with different chelating metals: nickel, copper, zinc and cobalt, so that a much wider range of proteins can be effectively separated. In addition, the chelates are offered at high or low density, where appropriate, so that the beads can deal effectively with different sizes of proteins, and make clean and effective separations.
ABT offers test kits with a range of chelates in small quantities so that you can try out your separation for a very low price, and then choose the best chelating beads to take forward.
The chelates have been very widely accepted by research labs and we have severer testimonials available.

“…We have since purchased 500 ml of ABT resin and have used this to purify several proteins in our lab. We have purified all-alpha helical and all-beta sheet proteins, from 100 – 300 amino acids in size, expressed in E.coli. We find that the ABT resin performs as well as that of other leading manufacturers but costs less. This has allowed us to make a significant saving as we work on many mutant proteins and therefore use a lot of pre-charged resin”.

Dept of Chemistry – University of Cambridge
Cambridge, Cambridgeshire CB2 1EW


Save the date

Join us, we will be happy to see you!

13th Annual Bioresearch Product Faire

May 16, 2012 at 10:00 AM – 2:30 PM.  University of Pennsylvania
Houston Hall, Hall of Flags and Bodek Lounge, 3417 Spruce St.,
Philadelphia, PA 19317.

Philadelphia will include new product demonstrations, scientific supplier exhibits, and hot topic seminars. This event is designed for active research scientists and related life science professionals. To speed up the registration process, please use the link to pre-register! Network with other Researchers, Learn Troubleshooting Skills, Connect with Industry Experts, Learn about Career Opportunities, View Product Demonstrations, Discover New Research Tools, Hear About Laboratory Services.

More information    Register

More show we will attend

June 21, 2012 – University of Pittsburgh, Pittsburgh PA
August 23, 2012 – University of California San Diego, San Diego CA
November 1, 2012 – University of Alabama Birmingham, Birmingham AL

Early summer sales

We have prepared a special offer for you.

Take advantage of our Early Summer Sales and save 30% on  all Gluthatione presentations.

This offer is valid until May 31st 2012.  Coupon GTS201230